10 Things to Consider When Buying dengue rapid test

Pinching the top with fingers and position the capillary tube into the specimen. Subsequently, lift the finger from the capillary tube slowly and fill the capillary tube with specimen. Make sure not to exceed the specimen line marked on the capillary tube. Then, hold the capillary tube vertically and pinch the top of capillary tube to dispense the entire specimen into the sample well (marked “S”).

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Pinching the bulb at the top with fingers and position the dropper into the specimen. Subsequently, lift the fingers slowly from the bulb to fill the dropper with the specimen. Then, hold the dropper vertically and pinch the bulb of the dropper to dispense 1 drop of serum/plasma, or 2 drops of whole blood (drop by drop) into the sample well (marked “S”). Make sure there are no air bubbles.

For video demo, please visit https://www.youtube.com/watch?v=kocxQLfMP8g

No. Buffer from other brand or other test kit cannot be used as a substitute for the buffer in this kit. Please contact your supplier for assistance. The buffers provided in the kit are more than the actual volume needed to complete all 25 tests in a kit. User should check the IFU again and ensure that the tests are run following the correct assay procedure.

It may be due to insufficient specimen or buffer volume. Sufficient amount of specimen and buffer are important to ensure the sample to flow up properly. Please repeat the test with a new test device if invalid results observed.

User should read the assay procedure in the IFU provided carefully before running the test. User should ensure that the correct volume of specimen is used during the testing.

• If using a micropipette, make sure 5 μl of specimen for IgG/IgM test, 60 μl of serum/plasma or 70 μl of whole blood for NS1 test are used.
• If using capillary tube for IgG/IgM test, make sure sample taken until the specimen line and dispense entire specimen into the well “S”
• If using dropper for NS1 Ag test, make sure 2 full drops of serum/plasma or 2 full drops of whole blood is dispensed into the well “S”
• For whole blood specimen, the sample pad area at well “S” will be fully covered with blood sample if the correct amount of specimen is added

When adding buffer, it is important to hold the buffer bottle vertically, carefully press the bottle to release full drop of buffer, one drop by one drop. The droplet volume of the buffer may be inconsistent if the buffer bottle is not hold vertically above the well “S”, or buffer being release too fast.

In rare cases, sample flow problem may also occur if too much specimen or buffer were added into well “S” or well “B”. Excessive sample may block the well and inhibit the sample flow to the sample pad.

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If coloured bands appear at the control line (C) and test lines (T, IgG, and/or IgM), it indicates a positive result. Positive results indicate the presence of Dengue NS1 antigen and Dengue antibodies in the specimen. Positive result should not be used as the sole criteria for the diagnosis of Dengue. Positives results should be confirmed by alternative testing method and clinical finding before positive determination is made.

If only control line (C) is visible, no coloured line developed at positions T, IgG or IgM. No IgG or IgM antibodies or NS1 antigen were detected. Negative result does not preclude the possibility of exposure to or infection with dengue viruses. Negative result can occur if the quantity of dengue NS1 antigen or antibody present in specimen is below the detection limit of the test, or the dengue antigen that are detected are not present during the stage of disease in which sample is collected.

Dengue is a flavivirus, transmitted by Aedesaegypti and Aedesalbopictus mosquitoes. It is widely distributed throughout the tropical and subtropical areas of the world, and causes up to 100 million infections annually. Classic Dengue infection is characterized by a sudden onset of fever, intense headache, myalgia, arthralgia and rash. NS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.

The Dengue NS1 Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid test that utilizes a combination of Dengue antibodies coated coloured particles for the detection of Dengue NS1 antigen in human whole blood, serum, or plasma.

Features and Benefits

• Tests for the viral infection Dengue, which is spread by mosquitoes
• Symptoms can include, high temperature, a severe headache, pain behind the eyes, muscle and joint pain, being or feeling sick, a widespread rash, loss of appetite
• The symptoms normally pass in a week, however you may feel tired and unwell for several weeks after

What you will receive

You will receive everything you need to carry out five tests.

• 10 x Test Devices
• 10 x Sample Diluent (Buffer solution)
• 10 x Pipettes (inside the pouch)
• 1 x Instructions For Use

This kit assumes you are a medical professional and already have a fingertip blood collection device (e.g. safety lancet).

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